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Documents can also be downloaded by choosing a. Filetype: pdf; Pages: 255. To send this document by email, select the number of copies you wish to download. IEC 61284 01.03 Fittings and attachments for general use.
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IEC 61284 is a standard laying out the requirements for circuit protection. Download pdf. View DOC .Chloride permeability of cultured rabbit intestinal epithelium.
Na+ absorption occurs more rapidly in the basolateral than the apical membrane of intestinal epithelia. To investigate whether this difference in Na+ absorption is due to Na+ transport being associated with Cl- transport in the basolateral membrane (BLM), Cl- transport across the BLM was studied in confluent monolayers of cultured rabbit intestinal epithelium. Monolayers were grown on filters and mounted in chambers. Basolateral Cl- efflux was estimated from the change in electrical resistance over time. Monolayers demonstrated a low basolateral Cl- conductance (BClt). Above a membrane potential (V(m)) of +30 mV, the basolateral Cl- conductance (BCls) progressively increased, reaching a V(m) of +90 mV after 24 hours. At the earliest time point BClt was 6 +/- 0.9 x 10(-14) cm2/sec (n = 43). This V(m) varied with the external [Cl-], reaching a maximum of 56 +/- 9 mV at 10 mM [Cl-]. BClt did not vary as the Cl- activity gradient in the basolateral bath was varied from 115 mM to 57 mM. Cis-inhibition of the Cl- channels by 1 mM 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS) reduced BClt, but BClt was restored after rinsing with DIDS-free solution. The results suggest that the basolateral membrane Cl- conduct
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